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Table 1 Specific interference with virus infection by bacterial crude preparations

From: Crude extracts of bacterially expressed dsRNA can be used to protect plants against virus infections

Bacterial preparation Phenotype Virus titer
Coinoculation (PPV)*   
HT115 S 3/3 1.221 ± 0.105
PPV HC dsRNA R 14/17 0.007 ± 0.004
  S 3/17 1.197 ± 0.060
PPV CP dsRNA R 8/11 0.017 ± 0.017
  S 3/11 1.310 ± 0.103
PMMoV IR 54 S 7/7 1.149 ± 0.078
Non-inoculated controls   0.012 ± 0.007
Spraying (PMMoV)   
PMMoV IR 54 (0 day) R 5/5 0.004 ± 0.002
PMMoV IR 54 (1 day) R 5/5 0.009 ± 0.003
PMMoV IR 54 (3 days) R 5/5 0.011 ± 0.010
PMMoV IR 54 (5 days) R 18/18 0.015 ± 0.012
PMMoV IR 54 (7 days) R 1/5 0.012
  S 4/5 1.360 ± 0.141
HT115 (5 days) S 10/10 1.420 ± 0.121
Non-inoculated controls   0.010 ± 0.009
Spraying (PPV)   
HT115 S 7/7 1.042 ± 0.260
PPV HC dsRNA R 11/12 0.004 ± 0.008
  S 1/12 0.278
Non-inoculated controls   0.008 ± 0.003
  1. *Plants were inoculated with mixtures of PPV plus French Press preparations derived from HT115 harboring either PPV HC dsRNA, PPV CP dsRNA, PMMoV IR 54 or the empty vector (HT115). The number of plants showing resistant (R) or susceptible (S) phenotypes to viral infection is indicated. Virus titer corresponds to the mean ELISA values ± SE at 12 dpi. PMMoV was inoculated on plants simultaneously or one, three, five or seven days after spraying with either PMMoV IR 54 or HT115 preparations in the same leaves. PPV was inoculated on plants five days after spraying with either PPV HC dsRNA or HT115 preparations in the same leaves.