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Table 1 Specific interference with virus infection by bacterial crude preparations

From: Crude extracts of bacterially expressed dsRNA can be used to protect plants against virus infections

Bacterial preparation

Phenotype

Virus titer

Coinoculation (PPV)*

  

HT115

S 3/3

1.221 ± 0.105

PPV HC dsRNA

R 14/17

0.007 ± 0.004

 

S 3/17

1.197 ± 0.060

PPV CP dsRNA

R 8/11

0.017 ± 0.017

 

S 3/11

1.310 ± 0.103

PMMoV IR 54

S 7/7

1.149 ± 0.078

Non-inoculated controls

 

0.012 ± 0.007

Spraying (PMMoV)

  

PMMoV IR 54 (0 day)

R 5/5

0.004 ± 0.002

PMMoV IR 54 (1 day)

R 5/5

0.009 ± 0.003

PMMoV IR 54 (3 days)

R 5/5

0.011 ± 0.010

PMMoV IR 54 (5 days)

R 18/18

0.015 ± 0.012

PMMoV IR 54 (7 days)

R 1/5

0.012

 

S 4/5

1.360 ± 0.141

HT115 (5 days)

S 10/10

1.420 ± 0.121

Non-inoculated controls

 

0.010 ± 0.009

Spraying (PPV)

  

HT115

S 7/7

1.042 ± 0.260

PPV HC dsRNA

R 11/12

0.004 ± 0.008

 

S 1/12

0.278

Non-inoculated controls

 

0.008 ± 0.003

  1. *Plants were inoculated with mixtures of PPV plus French Press preparations derived from HT115 harboring either PPV HC dsRNA, PPV CP dsRNA, PMMoV IR 54 or the empty vector (HT115). The number of plants showing resistant (R) or susceptible (S) phenotypes to viral infection is indicated. Virus titer corresponds to the mean ELISA values ± SE at 12 dpi. PMMoV was inoculated on plants simultaneously or one, three, five or seven days after spraying with either PMMoV IR 54 or HT115 preparations in the same leaves. PPV was inoculated on plants five days after spraying with either PPV HC dsRNA or HT115 preparations in the same leaves.