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Table 2 Accuracy and reproducibility of five different labeling methods in cDNA microarray hybridizations1.

From: Evaluation of five different cDNA labeling methods for microarrays using spike controls

cDNA labeling method

Relative deviation from expected values

Total CV2

Ratio of variability between and within arrays3

RAR4

CyScribe

0.16

0.38

2.09

0.17

FairPlay

0.36

0.22

1.49

0.20

TSA

0.48

0.45

1.78

0.68

3DNA

0.24

0.45

0.88

0.28

3DNA50

0.17

0.26

1.43

0.10

  1. 1The table shows only the overall values calculated as the median of ten individual values belonging to the ten spikes. 2CV is the total coefficient of variation of the ratio data. 3The values given are the square root of ratio between the treatment sum of squares (between arrays) and the error sum of squares (within array) from a one-way analysis of variance (see Methods). 4RAR is the relative accuracy and reproducibility (see Methods).
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BMC Biotechnology

ISSN: 1472-6750

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