Table 1 Expected and observed Cy5/Cy3 ratios for Arabidopsis spike controls in cDNA microarray hybridizations using five different labeling methods.
From: Evaluation of five different cDNA labeling methods for microarrays using spike controls
Spikes | Expected ratio | Observed ratio for the five methods1: | Range3 of MMR values | ||||
|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | |||
CyScribe [50 μg]2 | FairPlay [20 μg] | TSA [5 μg] | 3DNA [5 μg] | 3DNA50 [20 μg] | |||
Spike 7 | 0.125 | 0.16 (0.05)4 | 0.17 (0.05) | 0.10 (0.04) | 0.10 (0.06) | 0.17 (0.04) | 0.10 – 0.17 |
Spike 10 | 0.25 | 0.28 (0.09) | 0.44 (0.11) | 0.25 (0.11) | 0.26 (0.17) | 0.37 (0.10) | 0.25 – 0.44 |
Spike 9 | 0.33 | 0.25 (0.08) | 0.49 (0.18) | 0.27 (0.11) | 0.22 (0.13) | 0.27 (0.10) | 0.22 – 0.49 |
Spike 8 | 0.50 | 0.60 (0.21) | 0.67 (0.11) | 0.46 (0.14) | 0.45 (0.17) | 0.46 (0.11) | 0.45 – 0.67 |
Spike 1 | 1.00 | 1.22 (0.41) | 1.72 (0.28) | 1.69 (0.69) | 1.64 (0.83) | 2.42 (0.65) | 1.22 – 2.42 |
Spike 2 | 2.00 | 1.85 (0.70) | 2.66 (0.64) | 3.12 (1.20) | 2.40 (0.51) | 2.41 (0.53) | 1.85 – 2.41 |
Spike 3 | 3.00 | 1.56 (0.60) | 3.58 (1.36) | 4.20 (1.90) | 3.85 (0.70) | 3.44 (0.79) | 1.56 – 4.20 |
Spike 4 | 4.00 | 3.92 (1.34) | 4.85 (0.95) | 7.04 (2.77) | 5.15 (1.26) | 4.24 (1.06) | 3.92 – 7.04 |
Spike 5 | 5.00 | 5.63 (1.80) | 7.22 (1.39) | 13.99 (6.56) | 7.66 (1.25) | 5.78 (1.45) | 5.63 – 13.99 |
Spike 6 | 6.00 | 5.94 (2.04) | 7.97 (1.90) | 18.63 (10.64) | 5.38 (1.13) | 6.28 (1.16) | 5.38 – 18.63 |