Figure 7From: A new reverse transcription-polymerase chain reaction method for accurate quantificationApplication of the new quantitative RT-PCR to determine the ratios of gene expression among cell lines. The Collagen I cDNAs were synthesized by Omniscript reverse transcriptase. The observed 35-cycle values of long (L) over short (S) PCR products from different sample combinations were used for calculation of corrected ratios. 4N: wild-type VHL-transfected NRK; 6N: mutant VHL-transfected NRK.Back to article page