Figure 4
From: A new reverse transcription-polymerase chain reaction method for accurate quantification
Observed and baseline-corrected PCR product ratios (A) and efficiency plots (B). The 1:1, 5:1, and 10:1 volume ratios of long (L) to short (S) Collagen I cDNA synthesized by the Thermoscript reverse transcriptase were examined to determine the accuracy of corrected ratios (with value from either 1:1 or 5:1 ratio of corresponding PCR cycle as the denominator) to expected values.