Figure 3From: A new reverse transcription-polymerase chain reaction method for accurate quantificationObserved PCR product ratios (A) and PCR efficiency plots (B) of 1:1 VHL long to short cDNA. The long (with 10-base G or C linker) and short (VHL-S) cDNAs were synthesized by Thermoscript reverse transcriptase. Weak bands of much higher than the correct 123 bp and 113 bp products were single-stranded populations of these two amplicons.Back to article page