Figure 4
From: Expressing functional siRNAs in mammalian cells using convergent transcription
Suppression of p53 protein levels using a convergent U6 expression vector. (A) Transient suppression of p53 protein levels. Plasmids DualU6 and DualU6p53 or p53-specific siRNAs 1 and 2 were transfected into MDA MB 231 cells and, at 48 h and 120 h post-transfection, p53 and β-actin protein levels were determined using Western analysis. (B) Long term suppression of p53 protein levels. EcR293 cells containing a stably integrated dEGFP transgene were co-transfected with DualU6, DualU6GFP or DualU6p53 and pREP7. Following selection in hygromycin, cell populations were analysed for p53 and β-actin protein levels.