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Figure 2 | BMC Biotechnology

Figure 2

From: Expressing functional siRNAs in mammalian cells using convergent transcription

Figure 2

Gene suppression by complementary RNAs expressed from a U6 convergent cassette is Dicer-dependent. (A) The U6 convergent EGFP vector undergoes transcription in EcR293 cells to produce sense and antisense RNAs. (B) Suppression of target gene expression by the DualU6GFP vector requires the co-expression of both sense and antisense RNAs. (C) The DualU6GFP expression vector reduces dEGFP target gene expression in a Dicer-dependent manner. (D) Co-expression of complementary RNAs from the U6 convergent expression cassette does not activate PKR. Hela cells were treated with 0.1 μM calyculin A and serve as a positive control for activated PKR.

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