Table 2 Variance component estimates (standard error in parenthesis) for the three DNA quantification methods (four evaluations).
From: Performance of high-throughput DNA quantification methods
Variance Component | N (Replicates) | OD-U | OD-D | QG | PG |
|---|---|---|---|---|---|
Sample | 22 | 0.0064 (0.002) | 0.0067 (0.0021) | 0.0110 (0.0034) | 0.0130 (0.004) |
% of Total Variance | 95.7% | 60.0% | 55.0% | 36.0% | |
OD Aliquot | 4 | 0.0000 (0) | 0.0014 (0.0013) | 0.0005 (0.0005) | 0 (0) |
% of Total Variance | 0.8% | 12.8% | 2.3% | 0.0% | |
Dilution | 4 | N/A | 0.0005 (0.0002) | 0.0004 (0.0002) | 0.0011 (0.0004) |
% of Total Variance | Nested w/ OD Aliq. | 4.7% | 1.8% | 3.2% | |
Assay Aliquot | 16 | N/A | N/A | 0.0010 (0.0001) | 0.0004 (0.0001) |
% of Total Variance | 4.8% | 1.1% | |||
Run | 8 | 0.0000 (0) | 0 (0) | N/A | N/A |
% of Total Variance | 0.0% | 0.0% | |||
Residual Error | - | 0.0002 (0) | 0.0025 (0.0001) | 0.0072 (0.0001) | 0.0212 (0.0004) |
% of Total Variance | 3.2% | 22.8% | 36.0% | 59.4% | |
Total Variance | - | 0.0066 | 0.0111 | 0.0201 | 0.0357 |