TY - JOUR AU - Magin-Lachmann, Christine AU - Kotzamanis, George AU - D'Aiuto, Leonardo AU - Wagner, Ernst AU - Huxley, Clare PY - 2003 DA - 2003/02/03 TI - Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 – new vectors for in vitro and in vivodelivery JO - BMC Biotechnology SP - 2 VL - 3 IS - 1 AB - Bacterial artificial chromosomes (BACs) have been used extensively for sequencing the human and mouse genomes and are thus readily available for most genes. The large size of BACs means that they can generally carry intact genes with all the long range controlling elements that drive full levels of tissue-specific expression. For gene expression studies and gene therapy applications it is useful to be able to retrofit the BACs with selectable genes such as G418 resistance, reporter genes such as luciferase, and oriP/EBNA-1 from Epstein Barr virus which allows long term episomal maintenance in mammalian cells. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-3-2 DO - 10.1186/1472-6750-3-2 ID - Magin-Lachmann2003 ER -