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Table 2 Time animals take to reach 1 mm in length when CeMM is altered

From: Chemically defined medium and Caenorhabditis elegans

Condition 1,2

Days to reach 1 mm at 25°C 3

Fresh liquid CeMM

5

Fresh CeMM plate

5

Fresh liquid CeMM heated to 50°C for 1 h

5

Liquid CeMM conditioned 4.5 weeks, heated to 50°C for 1 h

4

Liquid CeMM conditioned 4.5 weeks, heated to 50°C for 1 h, filtered

8

Fresh liquid CeMM with added E. coli strain OP50

4

Liquid CeMM stored 1 y in the dark @ 4°C

5

Liquid CeMM stored 1 y in the dark @ 4°C, subject to periodic removal from storage for use

7–10

  1. 1 CeMM plates were 60 × 15 mm 2 Liquid CeMM was 200 μ l in a 24 well tissue culture dish 3 All times represent the average length of ten animals from three independent runs