Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Table 1 Real-time nucleotide primer sequences

					PRIMER CONCENTRATION ^&		PCR EFFICIENCY *
GENE	FORWARD PRIMER	REVERSE PRIMER	ACCESSION NUMBER	PRODUCT LENGTH	REFERNCE GENE	TARGET GENE	REFERNCE GENE	TARGET GENE
hGAPDH	AAC AGC GAC ACC CAT CCT C;	CAT ACC AGG AAA TGA GCT TGA CAA	NM_002046	81
CODING	CAC CTC TGG GCT ACG TGC TAG	GAA CAC ATG CTG AGG TTT AAA GAG AAT	NG_001332	98	50/50	300/300	-2.87	-2.84
SIGNAL	CAG AGG GGT GCC TCT GTC A	GGA CCC CTC ACA AAG TGT CG	NG_001332	142	100/100	100/100	-2.87	-2.93
C-MYC	TTC TAA GAG AAA TGT CCT GAG CAA TC	TCA AGA CTC AGC CAA GGT TGT G	X00364	88	100/100	500/500	-3.18	-3.22
GLI	CCC AAT CAC AAG TCA GGT TCC T	CCT ATG TGA AGC CCT ATT TGC C	NM_005269	93	100/100	300/300	-3.18	-3.19
N-MYC	CTT TCA AAT CGG TCC CCT GTC	AGA GGT TCC AGG GTC GTC TT	Y00664	82	50/50	300/300	-3.29	-3.30
OPA1	TGT GAT TGA AAA CAT CTA CCT TCC A	TTT AAG CTT GAT ATC CAC TGT GGT GT	NM_015560	82	50/50	500/500	-3.34	-3.38
MGAPDH	GTC TCC TGC GAC TTC AGC	TCA TTG TCA TAC CAG GAA ATG AGC	NM_008084	107
HOP	TGG AGT ACA ACT TCA ACA AGG TCA A	GGA CTC ACC TGC GTC TGC TC.	AF492703	103	50/50	300/300	-3.2	-3.18
FLAG/HOP	ATG GAC TAC AAG GAC GAT GAC CGA	TGT TGA CCT TGT TGA AGT TGT ACT CC	AF492703	107	50/50	100/100	-3.2	-3.21

^& Concentrations used in individual assay for F/R primers (nM). * PCR efficiency defined as the slope of the Log-linear phase of a dilution set as described in Figure 3A

ISSN: 1472-6750