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Table 1 Real-time nucleotide primer sequences

From: Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

      PRIMER CONCENTRATION & PCR EFFICIENCY *
GENE FORWARD PRIMER REVERSE PRIMER ACCESSION NUMBER PRODUCT LENGTH REFERNCE GENE TARGET GENE REFERNCE GENE TARGET GENE
hGAPDH AAC AGC GAC ACC CAT CCT C; CAT ACC AGG AAA TGA GCT TGA CAA NM_002046 81     
CODING CAC CTC TGG GCT ACG TGC TAG GAA CAC ATG CTG AGG TTT AAA GAG AAT NG_001332 98 50/50 300/300 -2.87 -2.84
SIGNAL CAG AGG GGT GCC TCT GTC A GGA CCC CTC ACA AAG TGT CG NG_001332 142 100/100 100/100 -2.87 -2.93
C-MYC TTC TAA GAG AAA TGT CCT GAG CAA TC TCA AGA CTC AGC CAA GGT TGT G X00364 88 100/100 500/500 -3.18 -3.22
GLI CCC AAT CAC AAG TCA GGT TCC T CCT ATG TGA AGC CCT ATT TGC C NM_005269 93 100/100 300/300 -3.18 -3.19
N-MYC CTT TCA AAT CGG TCC CCT GTC AGA GGT TCC AGG GTC GTC TT Y00664 82 50/50 300/300 -3.29 -3.30
OPA1 TGT GAT TGA AAA CAT CTA CCT TCC A TTT AAG CTT GAT ATC CAC TGT GGT GT NM_015560 82 50/50 500/500 -3.34 -3.38
MGAPDH GTC TCC TGC GAC TTC AGC TCA TTG TCA TAC CAG GAA ATG AGC NM_008084 107     
HOP TGG AGT ACA ACT TCA ACA AGG TCA A GGA CTC ACC TGC GTC TGC TC. AF492703 103 50/50 300/300 -3.2 -3.18
FLAG/HOP ATG GAC TAC AAG GAC GAT GAC CGA TGT TGA CCT TGT TGA AGT TGT ACT CC AF492703 107 50/50 100/100 -3.2 -3.21
  1. & Concentrations used in individual assay for F/R primers (nM). * PCR efficiency defined as the slope of the Log-linear phase of a dilution set as described in Figure 3A