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Table 1 Primer sequences used for template generation of the standard curves and real-time PCR amplification of hIL-5R and beta-actin genes

From: Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin – 5 receptor alpha spliced isoforms mRNA

 

mRNA target

Forward primer

Reverse primer

Amplicon size

Primer pair 1

Membrane-anchored hIL5Rα gemplate

standard curve

5'-GTGTCTGCTTTTCCAATCCATTG-3'

5'-TGCTGGAATTGGAAACAACT3'

347 bp

Primer pair 2

Soluble hIL5Rα template standard curve

5'-GTGTCTGCTTTTCCAATCCATTG-3'

5'-AATCTGCTATCCCTGCTGTTGTT-3'

294 bp

Primer pair 3

Membrane-anchored hIL5Rα

5'-GCAGCAGTGAGCTCCATGTG-3'

5'-AGGGCTTGTGTTCATCATTTCC 3'

89 bp

Primer pair 4

Soluble hIL5Rα

5'-GCAGCAGTGAGCTCCATGTG-3'

5'-TGGATGTTATCTCCTTTATCTTGAGAA-3'

95 bp

Primer pair 5

Beta-actin Template standard curve

5'CCAAGGCCAACCGCGAGAAGATGAC-3'

5'-AGGGTACATGGTGGTGCCGCCAGAC-3'

588 bp

Primer pair 6

Beta-actin

5'-CTGGAACGGTGAAGGTGACA-3'

5'-AAGGGACTTCCTGTAACAATGCA-3'

140 bp