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Table 1 Primer sequences used for template generation of the standard curves and real-time PCR amplification of hIL-5R and beta-actin genes

From: Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin – 5 receptor alpha spliced isoforms mRNA

  mRNA target Forward primer Reverse primer Amplicon size
Primer pair 1 Membrane-anchored hIL5Rα gemplate
standard curve
5'-GTGTCTGCTTTTCCAATCCATTG-3' 5'-TGCTGGAATTGGAAACAACT3' 347 bp
Primer pair 2 Soluble hIL5Rα template standard curve 5'-GTGTCTGCTTTTCCAATCCATTG-3' 5'-AATCTGCTATCCCTGCTGTTGTT-3' 294 bp
Primer pair 3 Membrane-anchored hIL5Rα 5'-GCAGCAGTGAGCTCCATGTG-3' 5'-AGGGCTTGTGTTCATCATTTCC 3' 89 bp
Primer pair 4 Soluble hIL5Rα 5'-GCAGCAGTGAGCTCCATGTG-3' 5'-TGGATGTTATCTCCTTTATCTTGAGAA-3' 95 bp
Primer pair 5 Beta-actin Template standard curve 5'CCAAGGCCAACCGCGAGAAGATGAC-3' 5'-AGGGTACATGGTGGTGCCGCCAGAC-3' 588 bp
Primer pair 6 Beta-actin 5'-CTGGAACGGTGAAGGTGACA-3' 5'-AAGGGACTTCCTGTAACAATGCA-3' 140 bp