mRNA target | Forward primer | Reverse primer | Amplicon size | |
---|---|---|---|---|
Primer pair 1 | Membrane-anchored hIL5Rα gemplate standard curve | 5'-GTGTCTGCTTTTCCAATCCATTG-3' | 5'-TGCTGGAATTGGAAACAACT3' | 347 bp |
Primer pair 2 | Soluble hIL5Rα template standard curve | 5'-GTGTCTGCTTTTCCAATCCATTG-3' | 5'-AATCTGCTATCCCTGCTGTTGTT-3' | 294 bp |
Primer pair 3 | Membrane-anchored hIL5Rα | 5'-GCAGCAGTGAGCTCCATGTG-3' | 5'-AGGGCTTGTGTTCATCATTTCC 3' | 89 bp |
Primer pair 4 | Soluble hIL5Rα | 5'-GCAGCAGTGAGCTCCATGTG-3' | 5'-TGGATGTTATCTCCTTTATCTTGAGAA-3' | 95 bp |
Primer pair 5 | Beta-actin Template standard curve | 5'CCAAGGCCAACCGCGAGAAGATGAC-3' | 5'-AGGGTACATGGTGGTGCCGCCAGAC-3' | 588 bp |
Primer pair 6 | Beta-actin | 5'-CTGGAACGGTGAAGGTGACA-3' | 5'-AAGGGACTTCCTGTAACAATGCA-3' | 140 bp |