GFP-specific sequences in rat lung at 12 months of age. DNA was extracted from rat lungs. Undigested DNA (10 μg) was electrophoresed on agarose gel for Southern blot analysis. DNA from rAAV2gfp infected 293 cells was used as a positive control. Lanes labeled AAV-GFP represents DNA from separate animals infected at 16 days gestation with rAAV2gfp. Negative Control labeled lanes are from age-matched animals not infected with an rAAV2.