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Figure 6 | BMC Biotechnology

Figure 6

From: Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties

Figure 6

Complexation of plasmid DNA by IBB peptide. Complexation was allowed to proceed 15 min at room temperature in 50 mM Hepes buffer, pH 7.5. The migration of DNA was analyzed on a 0.8 % agarose gel stained with ethidium bromide. Lane 1: supercoiled DNA ladder (Gibco BRL), lane 2 and 8: pXL3031 plasmid (3700 bp), lane 3 to 7: IBB peptide / pXL3031 complexes at molar ratio of 100 (3), 250 (4), 500 (5), 1000 (6) and 2000 (7).

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BMC Biotechnology

ISSN: 1472-6750

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