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Figure 5 | BMC Biotechnology

Figure 5

From: Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties

Figure 5

Transfection of HeLa cells using a ternary lipoplex. IBB peptide / plasmid / RPR120535 cationic lipid lipoplexes were prepared in 150 mM NaCl and diluted with serum-containing cell culture medium. The peptide was electrostatically associated to pXL3031 luciferase-encoding plasmid at a ratio varying from 0 to 500 IBB peptide / plasmid (mol / mol). Luciferase expression was evaluated 24 h after transfection.

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BMC Biotechnology

ISSN: 1472-6750

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