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Figure 1 | BMC Biotechnology

Figure 1

From: Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties

Figure 1

Transfection of HeLa cells using a ternary lipoplexe IBB peptide / plasmid / RPR120535 cationic lipid. Lipoplexes were prepared in bicarbonate buffer (150 mM NaCl, 20 mM NaHCO3, pH 10) and diluted with serum-containing cell culture medium. The peptide was covalently coupled (covalent) or electrostatically associated (electrostatic) to pXL3031 luciferase-encoding plasmid at a ratio varying from 0 to 500 IBB peptide / plasmid (mol / mol). Luciferase expression was evaluated 24 h after transfection.

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