Figure 6

The RNA from microneedle collected cells was extracted and amplification was performed using SMART PCR. Separately, 1μg of Universal Mouse Reference RNA (Stratagene) were used to generate reference cDNA. Fig 6A shows a gel picture of amplified cDNA from microneedle collected cells. The numbers indicate the PCR cycle number. The generated PCR product from the needle sample as well as the Universal Mouse Reference cDNA were labeled with Cy5 or Cy3. The two resulting cDNA probes were then mixed together and hybridized to a microarray slide containing 9700 genes. Fig 6B shows a part of the entire array (4 out of 16 blocks) generated using PCR amplified probe. Fig 6C is a histogram of the distribution of gene expression (CY5/Cy3) ratios for the array elements for hybridizations comparing gene expression.