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Figure 3 | BMC Biotechnology

Figure 3

From: Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse

Figure 3

The correlation coefficient demonstrates that amplified cDNA is comparable to that of conventionally isolated RNA from large numbers of cells. A) By comparing the distribution of ratios of gene expression in MTLn3 and MTC, the relatedness of the expression patterns of the two cell types is establish on a 9000 gene chip. Log base 2 ratios from 2 chips were plotted for unamplified probes from MTLn3 and MTC cells. B) Log base 2 ratios from 2 chips using PCR amplified probes of MTLn3 and MTC cells shows a higher correlation coefficient. C) The mean of the log base 2 ratios in A and B shows a correlation coefficient within the accepted range for conventional RNA methods.

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