Figure 2From: Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouseRelative abundance of genes is maintained during cDNA amplification as verified by Quantitative real-time PCR. cDNA from 1000 MTLn3 (a metastatic cell line) or 1000 MTC cells (non-metastatic cell line) was diluted (1:10) after 20 cycles of SMART PCR amplification, and the expression of selected genes was compared with cDNA from 3 μg unamplified total RNA from MTLn3 or MTC cells by real-time PCR.Back to article page