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Figure 2 | BMC Biotechnology

Figure 2

From: Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse

Figure 2

Relative abundance of genes is maintained during cDNA amplification as verified by Quantitative real-time PCR. cDNA from 1000 MTLn3 (a metastatic cell line) or 1000 MTC cells (non-metastatic cell line) was diluted (1:10) after 20 cycles of SMART PCR amplification, and the expression of selected genes was compared with cDNA from 3 μg unamplified total RNA from MTLn3 or MTC cells by real-time PCR.

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