Figure 5

Denaturing elution from glutathione agarose. Glutathione agarose beads from the purifications of the expression clones clone 1, 3a, 9a, 11a, 13a and 14a (see Figure 4) were washed with wash buffer after glutathione elution. A second elution was performed under denaturing conditions with SDS-PAGE sample buffer to elute proteins that could not be eluted with glutathione. The first lane per clone represents the whole cellular extract and the second lane the purified protein.