Figure 4

Comparison of the yields of His-tag and GST-tag purification. 1 ml aliquots of a 100 ml culture of expression clone 1, 3a, 9a, 11a, 13a and 14a were distributed in two deep well plates, 8 wells/plate. Cells were pelleted and the target proteins of one plate were purified with Ni-NTA-agarose and those of the other plate with glutathione agarose. The obtained protein yields are shown here.