Figure 2

48 purifications of CGI-114-protein. (A) Coomassie stained SDS-PAGE of 8 out of the 48 purifications of the CGI-114-protein, M = molecular weight standard, W = cellular protein, P = purified protein. 1 ml aliquots of a 100 ml culture expressing the CGI-114-protein were distributed in 48 wells of a deep well plate. Cells were pelleted and recombinant proteins purified with Ni-NTA agarose. (B) Distribution of protein purification yields.