Table 1 Primers used to polony amplify p53 and K-ras 2 exons bearing mutational hotspots from pancreatic cancer cell line genomic DNA.
Primer Name | Sequence |
|---|---|
p53 exon5 forward | tgccctgactttcaactctgtctccttcctc |
p53 exon5 reverse | ccagacctaagagcaatcagtgaggaatcagaggc |
p53 exon7 forward | gttatctcctaggttggctctgactgtacca |
p53 exon7 reverse | gtggatgggtagtagtatggaagaaatcggt |
p53 exon8 forward | ggtaggacctgatttccttactgcctcttgc |
p53 exon8 reverse | gataaaagtgaatctgaggcataactgcacc |
kras exon1 forward | tggtggagtatttgatagtgtattaaccttatgtg |
kras exon1 reverse | agagaaacctttatctgatatcaaagaatggtcctg |
kras exon2 forward | tgaagtaaaaggtgcactgtaataatccagac |
kras exon2 reverse | taatgtcagcttattatattcaatttaaacccacc |