Table 4 Permeation of the pellets by freezing at -75°C. Three Phase Partitioning (TPP) method applied to the pelleted culture "I" (samples 1, 2, 3, 4), culture "J" (samples 5, 6, 7, 8, 9, 10), directly (TPP) or following sonication of the pellets (S+TPP).
Pellet | Cycle | Final | BSA | gfp uv | gfp uv | Total | Specific mass |
|---|---|---|---|---|---|---|---|
Sample | BSA (mg/mL) | yield (%) | (μg/mL) | yield (%) | gfp uv (μg/mL) | gfp uv /BSA (μg/mg) | |
1 | (-75°C) | 31.49 | 62.05 | 337.91 | 57.50 | 10.73 | |
1st S+TPP | 5.34 | 10.53 | 137.52 | 23.40 | 25.74 | ||
2nd S+TPP | 13.92 | 27.42 | 112.28 | 19.11 | 587.71 | 8.07 | |
2 | (-75°C) | 45.35 | 68.04 | 358.35 | 58.12 | 7.90 | |
1st S+TPP | 5.82 | 87.30 | 165.33 | 26.82 | 28.39 | ||
2nd S+TPP | 15.47 | 23.21 | 92.854 | 15.06 | 616.53 | 6.00 | |
3 | (-75°C) | 10.87 | 28.78 | 91.75 | 23.41 | 8.42 | |
1st TPP | 4.01 | 10.65 | 194.30 | 49.67 | 48.41 | ||
2nd S+TPP | 22.89 | 60.59 | 105.34 | 26.93 | 391.20 | 4.60 | |
4 | (-75°C) | 8.71 | 46.03 | 73.207 | 26.85 | 8.40 | |
1st TPP | 2.96 | 15.65 | 134.53 | 49.34 | 45.39 | ||
2nd S+TPP | 7.25 | 38.30 | 64.94 | 23.82 | 272.68 | 8.96 | |
5 | (-75°C) | 75.14 | 66.45 | 1633.78 | 46.89 | 21.74 | |
1st S+TPP | 12.12 | 10.72 | 645.28 | 18.52 | 53.24 | ||
2nd S+TPP | 14.82 | 13.10 | 929.12 | 26.67 | 62.69 | ||
3rd S+TPP | 10.99 | 9.72 | 275.78 | 7.92 | 3483.96 | 25.10 | |
6 | (-75°C) | 35.54 | 49.78 | 1262.9 | 37.08 | 35.53 | |
1st S+TPP | 11.74 | 16.44 | 998.66 | 29.32 | 85.07 | ||
2nd S+TPP | 13.99 | 19.56 | 873.14 | 25.64 | 62.41 | ||
3rd S+TPP | 10.11 | 14.16 | 271.34 | 7.97 | 3406.04 | 26.84 | |
7 | (-75°C) | 52.66 | 60.55 | 1606.06 | 37.88 | 30.50 | |
1st S+TPP | 16.45 | 18.91 | 1528.08 | 36.04 | 92.90 | ||
2nd S+TPP | 13.25 | 15.23 | 875.68 | 20.65 | 66.09 | ||
3rd S+TPP | 4.60 | 5.29 | 230.03 | 5.43 | 4239.85 | 49.97 | |
8 | (-75°C) | 14.77 | 32.50 | 584.69 | 25.87 | 39.60 | |
1st TPP | 7.76 | 17.08 | 581.31 | 25.72 | 74.90 | ||
2nd TPP | 14.94 | 32.88 | 811.9 | 35.92 | 54.35 | ||
3rd S+TPP | 7.96 | 17.53 | 282.44 | 12.50 | 1977.90 | 35.46 | |
9 | (-75°C) | 18.41 | 34.54 | 721.81 | 21.39 | 39.21 | |
1st TPP | 12.62 | 23.68 | 1199.2 | 35.54 | 95.03 | ||
2nd TPP | 9.22 | 17.29 | 921.12 | 27.30 | 99.95 | ||
3rd S+TPP | 13.04 | 24.47 | 531.49 | 15.75 | 2842.13 | 40.75 | |
10 | (-75°C) | 15.20 | 27.80 | 567.94 | 17.88 | 37.36 | |
1st TPP | 13.23 | 24.18 | 1461.90 | 46.03 | 110.47 | ||
2nd TPP | 9.14 | 16.70 | 699.6 | 22.02 | 76.53 | ||
3rd S+TPP | 17.13 | 31.31 | 446.35 | 14.02 | 2729.42 | 26.05 |