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Table 4 Permeation of the pellets by freezing at -75°C. Three Phase Partitioning (TPP) method applied to the pelleted culture "I" (samples 1, 2, 3, 4), culture "J" (samples 5, 6, 7, 8, 9, 10), directly (TPP) or following sonication of the pellets (S+TPP).

From: Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp uv ) from Escherichia coli

Pellet Cycle Final BSA gfp uv gfp uv Total Specific mass
Sample   BSA (mg/mL) yield (%) (μg/mL) yield (%) gfp uv (μg/mL) gfp uv /BSA (μg/mg)
1 (-75°C) 31.49 62.05 337.91 57.50   10.73
  1st S+TPP 5.34 10.53 137.52 23.40   25.74
  2nd S+TPP 13.92 27.42 112.28 19.11 587.71 8.07
2 (-75°C) 45.35 68.04 358.35 58.12   7.90
  1st S+TPP 5.82 87.30 165.33 26.82   28.39
  2nd S+TPP 15.47 23.21 92.854 15.06 616.53 6.00
3 (-75°C) 10.87 28.78 91.75 23.41   8.42
  1st TPP 4.01 10.65 194.30 49.67   48.41
  2nd S+TPP 22.89 60.59 105.34 26.93 391.20 4.60
4 (-75°C) 8.71 46.03 73.207 26.85   8.40
  1st TPP 2.96 15.65 134.53 49.34   45.39
  2nd S+TPP 7.25 38.30 64.94 23.82 272.68 8.96
5 (-75°C) 75.14 66.45 1633.78 46.89   21.74
  1st S+TPP 12.12 10.72 645.28 18.52   53.24
  2nd S+TPP 14.82 13.10 929.12 26.67   62.69
  3rd S+TPP 10.99 9.72 275.78 7.92 3483.96 25.10
6 (-75°C) 35.54 49.78 1262.9 37.08   35.53
  1st S+TPP 11.74 16.44 998.66 29.32   85.07
  2nd S+TPP 13.99 19.56 873.14 25.64   62.41
  3rd S+TPP 10.11 14.16 271.34 7.97 3406.04 26.84
7 (-75°C) 52.66 60.55 1606.06 37.88   30.50
  1st S+TPP 16.45 18.91 1528.08 36.04   92.90
  2nd S+TPP 13.25 15.23 875.68 20.65   66.09
  3rd S+TPP 4.60 5.29 230.03 5.43 4239.85 49.97
8 (-75°C) 14.77 32.50 584.69 25.87   39.60
  1st TPP 7.76 17.08 581.31 25.72   74.90
  2nd TPP 14.94 32.88 811.9 35.92   54.35
  3rd S+TPP 7.96 17.53 282.44 12.50 1977.90 35.46
9 (-75°C) 18.41 34.54 721.81 21.39   39.21
  1st TPP 12.62 23.68 1199.2 35.54   95.03
  2nd TPP 9.22 17.29 921.12 27.30   99.95
  3rd S+TPP 13.04 24.47 531.49 15.75 2842.13 40.75
10 (-75°C) 15.20 27.80 567.94 17.88   37.36
  1st TPP 13.23 24.18 1461.90 46.03   110.47
  2nd TPP 9.14 16.70 699.6 22.02   76.53
  3rd S+TPP 17.13 31.31 446.35 14.02 2729.42 26.05