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Table 2 Three-phase-partitioning (TPP) method applied directly to cells of E. coli with specific growth rate constant (μ, h-1): of 0.99 h-1 for culture A, 1.05 h-1 for cultures B, C, D, E and of 1.10 h-1 for cultures F, G, H.

From: Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp uv ) from Escherichia coli

  TPP (1)
Sample gfp uv (μg/Ml) BSA (mg/mL) gfp uv /BSA (μg/mg)
A1 354.08 3.30 107.28
A2 444.05 3.29 135.10
A (Pool) 378.53 3.98 95.11
B1 46.68 2.60 18.05
B2 38.59 2.24 17.26
B (Pool) 45.66 2.42 18.87
C 39.60 1.11 35.65
D 15.92 0.41 38.86
E 28.12 0.73 38.70
F1 15.78 0.52 30.42
F2 11.56 0.22 52.69
F (Pool) 5.47 0.67 8.12
G 19.42 0.79 24.53
H1 16.69 0.82 20.44
H2 9.16 0.19 47.67
H 4.78 0.67 7.09