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Table 2 Three-phase-partitioning (TPP) method applied directly to cells of E. coli with specific growth rate constant (μ, h-1): of 0.99 h-1 for culture A, 1.05 h-1 for cultures B, C, D, E and of 1.10 h-1 for cultures F, G, H.

From: Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp uv ) from Escherichia coli

 

TPP (1)

Sample

gfp uv (μg/Ml)

BSA (mg/mL)

gfp uv /BSA (μg/mg)

A1

354.08

3.30

107.28

A2

444.05

3.29

135.10

A (Pool)

378.53

3.98

95.11

B1

46.68

2.60

18.05

B2

38.59

2.24

17.26

B (Pool)

45.66

2.42

18.87

C

39.60

1.11

35.65

D

15.92

0.41

38.86

E

28.12

0.73

38.70

F1

15.78

0.52

30.42

F2

11.56

0.22

52.69

F (Pool)

5.47

0.67

8.12

G

19.42

0.79

24.53

H1

16.69

0.82

20.44

H2

9.16

0.19

47.67

H

4.78

0.67

7.09