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Figure 3 | BMC Biotechnology

Figure 3

From: Glycerol restores heat-induced p53-dependent apoptosis of human glioblastoma cells bearing mutant p53

Figure 3

Gel mobility-shift assay of nuclear extracts or whole cell extracts from A-172/neo or A-172/mp53/248 cells to p53CON. in vivo, the cells were treated with glycerol (0.6 M), wortmannin (20 μM) and heat (at 44°C for 30 min). The nuclear fraction was extracted from the treated cells 6 h after heating. in vitro, the whole cell extracts prepared from intact cells, and then they were treated with glycerol, wortmannin and heat.

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