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Table 2 Summary of transgenic pig analysis.

From: Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.

   F0 Generation F1 Generation
Animals (F0) Strain Sex Southern* (Tail) Southern* (Sperm) SEAP Assay** Southern (Ratio) Positive Pigs Littermate
1 D M -   +    
2 D M -   -    
3 D M -   +++    
4 D M -   - 7/16 148–150, 152, 153, 155, 474 148–155, 469–476
5 D M + - -    
6 D F -   +    
7 D F -   -    
8 Y M - - - 1/5 187 184–188
9 Y M - - -    
10 Y M - - +    
11 Y M - - + 0/7   138–144
12 Y M - - -    
13 Y F -   NA    
14 Y F -   + 0/12   460–468, 477–479
15 Y F -   +++    
16 Y M -   +++    
17 Y M +   +++    
18 Y F -   - 0/10   401–410
19 Y M -   +++    
20 Y M - - +    
21 Y M - - +    
22 Y F -   ++ 0/12   215–226
23 Y F -   +++ 2/15 418, 419 411–425
24 Y M -   ++    
25 Y M - + +++ 0/3   198–200
26 Y F +   ++ 0/10   145, 430–438
27 Y F -   NA    
28 Y F -   NA 0/8   381–388
29 Y F -   - 0/2   166, 167
30 Y M - + -    
31 Y M -   ++ 7/30 441, 442, 448, 451, 452, 455, 457 439–459, 480–488
32 Y F -   NA    
33 Y F -   + 0/6   389–394
34 Y M - - -    
35 Y M - - +++    
36 L F +   NA    
37 L F -   NA    
38 L M -   -    
39 L M -   -    
40 L M +   NA    
41 L F -   - 0/9   175–182, 197
42 L F +   - 1/5 156 156–160
43 L F +   ++ 1/9 206 201–209
44 D M +   +    
45 D M -   -    
46 D F -   +    
47 D F -   NA    
48 D F +   +    
49 D F -   +++    
50 D F -   +++    
51 D M -   ++    
52 D M -   ++    
53 D M -   +++    
54 D M -   NA    
55 D F -   +    
56 D F -   +    
57 D F -   -    
58 D F -   ++    
59 D M -   +++    
60 L F -   NA    
61 L M -   NA    
62 L F -   NA    
63 L M -   NA    
64 L M +   NA    
65 L F -   -    
66 L F -   NA    
67 L F -   NA    
68 L M -   -    
69 L M -   -    
70 L M -   ++    
71 L F -   -    
72 L M -   NA    
73 L F -   -    
74 L M -   -    
75 L M -   +    
  1. D: Duroc; Y: Yorkshire; L: Landrace. *: + or - indicated detectable or undetectable hybridization signal in Southern blot analysis. **: - represents SEAP enzyme activity less than 2 × 104 RLU; + represents SEAP enzyme activity between 2–4 × 104 RLU; ++ represents SEAP enzyme between 4–6 × 104 RLU; +++ represents SEAP activity more than 6 × 104 RLU.