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Table 4 Partitioning of cell-linked and extracellular COX after phase-separation of Triton X-114

From: Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration

   COX total units COX activity (U/ml) K
% Triton X-114 % volume of rich phase Rich phase Depleted phase Rich phase Depleted phase  
Cell-linked       
1 10.8 8.5 234.8 78.7 263.2 0.30
2 15.9 137.9 136.5 867.3 162.3 5.34
3 29.6 276.5 87.1 934.1 123.7 7.55
4 37.5 278.0 86.5 741.3 138.4 5.35
6 41.8 280.0 85.0 669.8 146.0 4.58
Extracellular       
1 6.4 5.0 113.7 78.2 121.5 0.64
2 9.3 22.8 99.7 245.7 109.7 2.24
3 10.7 58.8 69.4 550.5 77.7 7.08
4 12.8 70.5 55.1 552.3 63.1 8.75
6a 15.3 84.0 41 547.9 48.4 11.32
6 15.3 390 41 2544 48.4 52.60
  1. aCOX units in rich phase calculated as if no activation occurred: total units before partitioning (125) – units in depleted phase (41)