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Table 3 Purification and concentration of COX during Triton X-114 phase separation.

From: Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration

 

Cells extract of 3%Triton X-114 w/v

Culture broth with 6% Triton X-114 w/v

Purification fold

1.67

11.6(2.52)b

Concentration factor

2.56

20.3 (4.38)b

% recovery

76 (70)a

312 (65)b

  1. aRecovery in this step and, in parenthesis, with respect to cells. b Figures in parenthesis correspond to purification, concentration and recovery in this step as if no activation occurred, calculated on the basis of remaining activity in the upper detergent-depleted phase