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Table 1 Effect of the cholesterol emuIsification method on the production of COX.

From: Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration

 

COX activity (U/ml)*

 

Emulsification cholesterol method

Cell-linked

extracellular

Dry weight (mg/ml)

Spray-dry

230

140

8.75

At the flame

60

60

9.05

Improvement

3.8

2.3

0.97

  1. *Enzymatic activity figures correspond to 70 hours of fermentation.