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Figure 4 | BMC Biotechnology

Figure 4

From: Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration

Figure 4

SDS-PAGE of COX fractions using 3% Triton X-114 for extraction, purification and concentration, (a) Cell extracts: lane 1, Mw markers; lane 2, commercial COX; lane 3, total extracted proteins; lane 4, proteins in detergent depleted phase; lane 5, proteins in detergent rich phase, (b) Culture broth: lane 1, Mw markers; lane 2, commercial COX; lane 3, proteins in detergent rich phase; lane 4, total proteins in culture broth; lane 5, proteins in detergent depleted phase. Arrows indicated the COX band.

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