Table 1 Relative in vitro splicing activity of constructs containing anti-theophylline aptamers. Extent of reaction is relative to the parental construct in 3 mM MgCl2 with no theophylline at 2 hrs.
[Mg Cl2] | 3 mM | 8 mM | ||
|---|---|---|---|---|
[Theo] | 1.5 mM | 0 mM | 1.5 mM | 0 mM |
Parental | 0.85 | 1 | 0.61 | 0.68 |
B11 | 0.03 | 0.02 | 0.31 | 0.34 |
Th1P6 | 0.2 | 0.05 | 0.31 | 0.16 |
Th2P6 | 0.15 | 0.04 | 0.31 | 0.04 |
Th3P6 | 0.03 | 0.04 | 0.2 | 0.04 |
Th4P6 | 0.06 | 0.05 | 0.38 | 0.37 |
Th5P6 | 0.04 | 0 | 0.05 | 0.03 |
Th6P6 | 0.03 | 0.01 | 0 | 0.03 |
Th1P5 | 1.08 | 0.91 | 0.85 | 0.74 |
Th1P5 | 0.03 | 0.03 | 0.7 | 0.57 |