Skip to main content

Advertisement

Table 5 Primers and probes used for quantitative real-time PCR assays. Primers and TaqMan probes were designed on the GenBank sequences indicated. Probes were labelled with FAM and TAMRA at the 5' and 3' ends, respectively.

From: Estimating the number of integrations in transformed plants by quantitative real-time PCR

a px gene, tomato endogenous (93 bp amplicon) (from GenBank acc. no. Y16773)
Q-TOMAPX-2818(+) 5'-TTTTACTTGTATATTCGAAGTGTGCCA-3'
Q-TOMAPX-2910(-) 5'-ACAACTGCAAAATTAGAATCTAGTTGGTA-3'
Q-TOMATO probe 5'-TTTCACCTATCTGGACCAAGTTTTGGCAAG-3'
TSWV-N transgene (79 bp amplicon) (from GenBank acc. no. Z36882)
Q-TSWVN-492(+) 5'-TGTCTTGGCTATATATCAGGATGCA-3'
Q-TSWVN-570(-) 5'-TAAGGCTTCCCTGGTGTCATACTT-3'
Q-TSWVN probe 5'-ACAAGGACCTTGGGATCGACCCAAA-3'
nptII transgene (60 bp amplicon) (from GenBank acc. no. U09365)
Q-NPT-250(+) 5'-AGGAAGCGGTCAGCCCAT-3'
Q-NPT-309(-) 5'-GCGTTGGCTACCCGTGATAT-3'
Q-NPT probe 5'-CGCCGCCAAGCTCTTCAGCAA-3'