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Table 5 Primers and probes used for quantitative real-time PCR assays. Primers and TaqMan probes were designed on the GenBank sequences indicated. Probes were labelled with FAM and TAMRA at the 5' and 3' ends, respectively.

From: Estimating the number of integrations in transformed plants by quantitative real-time PCR

a px gene, tomato endogenous (93 bp amplicon)

(from GenBank acc. no. Y16773)

Q-TOMAPX-2818(+)

5'-TTTTACTTGTATATTCGAAGTGTGCCA-3'

Q-TOMAPX-2910(-)

5'-ACAACTGCAAAATTAGAATCTAGTTGGTA-3'

Q-TOMATO probe

5'-TTTCACCTATCTGGACCAAGTTTTGGCAAG-3'

TSWV-N transgene (79 bp amplicon)

(from GenBank acc. no. Z36882)

Q-TSWVN-492(+)

5'-TGTCTTGGCTATATATCAGGATGCA-3'

Q-TSWVN-570(-)

5'-TAAGGCTTCCCTGGTGTCATACTT-3'

Q-TSWVN probe

5'-ACAAGGACCTTGGGATCGACCCAAA-3'

nptII transgene (60 bp amplicon)

(from GenBank acc. no. U09365)

Q-NPT-250(+)

5'-AGGAAGCGGTCAGCCCAT-3'

Q-NPT-309(-)

5'-GCGTTGGCTACCCGTGATAT-3'

Q-NPT probe

5'-CGCCGCCAAGCTCTTCAGCAA-3'