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Table 4 Comparison between copy number values as estimated by real-time PCR and Southern blotting. The results of estimates of TSWV-N transgene copy number obtained with real-time PCR are compared with those derived from Southern analysis, either limited to one restriction enzyme (KpnI) or extended to several ones.

From: Estimating the number of integrations in transformed plants by quantitative real-time PCR

Line real-time PCR Southern (KpnI) Southern (extended)
111–6 1 1 1
99–1 1 2 1–2
1–2 c 1 1 1–2
110–1 2 1 1–2
46–1 2 1 1–2
80–1 2 1 1–2
127–1 2 4 4–7
133–1 3 2 2–3
1–1 3 n.t.* n.t.
30–4 3–4 1 1–4
113–12 3–4 2 2–4
20–2 7–10 4 4–8
197–1 7–11 4–6 3–6
118–2 0 0 0
Hom.110–1 2 1 1–2
  1. * not tested