Table 4 Comparison between copy number values as estimated by real-time PCR and Southern blotting. The results of estimates of TSWV-N transgene copy number obtained with real-time PCR are compared with those derived from Southern analysis, either limited to one restriction enzyme (KpnI) or extended to several ones.
From: Estimating the number of integrations in transformed plants by quantitative real-time PCR
Line | real-time PCR | Southern (KpnI) | Southern (extended) |
|---|---|---|---|
111–6 | 1 | 1 | 1 |
99–1 | 1 | 2 | 1–2 |
1–2 c | 1 | 1 | 1–2 |
110–1 | 2 | 1 | 1–2 |
46–1 | 2 | 1 | 1–2 |
80–1 | 2 | 1 | 1–2 |
127–1 | 2 | 4 | 4–7 |
133–1 | 3 | 2 | 2–3 |
1–1 | 3 | n.t.* | n.t. |
30–4 | 3–4 | 1 | 1–4 |
113–12 | 3–4 | 2 | 2–4 |
20–2 | 7–10 | 4 | 4–8 |
197–1 | 7–11 | 4–6 | 3–6 |
118–2 | 0 | 0 | 0 |
Hom.110–1 | 2 | 1 | 1–2 |