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Table 2 Calculated copy number for TSWV-N and nptII transgenes. For each line and for each gene system, the r line ratio (SQ trans /SQ end ) is shown together with its 95% confidence interval (δr line ) in the left column. The calculated copy number, obtained as the ratio between r line and the virtual calibrator r1 is shown in the right column, together with its 95% confidence interval.

From: Estimating the number of integrations in transformed plants by quantitative real-time PCR

 

TSWV-N

nptII

Line

r line

r line /r1

r line

r line /r1

111–6

0.23 ± 0.04

1.10 ± 0.19

0.34 ± 0.05

1.10 ± 0.16

99–1

0.22 ± 0.05

1.05 ± 0.24

0.34 ± 0.07

1.10 ± 0.23

1–2 c

0.23 ± 0.04

1.10 ± 0.19

0.59 ± 0.05

1.90 ± 0.16

110–1

0.47 ± 0.07

2.24 ± 0.33

0.72 ± 0.06

2.32 ± 0.19

46–1

0.36 ± 0.12

1.71 ± 0.57

0.24 ± 0.09

0.77 ± 0.29

80–1

0.39 ± 0.05

1.86 ± 0.24

0.76 ± 0.13

2.45 ± 0.42

127–1

0.47 ± 0.11

2.24 ± 0.52

1.16 ± 0.13

3.74 ± 0.42

133–1

0.72 ± 0.10

3.43 ± 0.48

0.87 ± 0.09

2.81 ± 0.29

1–1

0.60 ± 0.13

2.86 ± 0.62

0.64 ± 0.12

2.06 ± 0.39

30–4

0.77 ± 0.19

3.67 ± 0.90

0.84 ± 0.19

2.71 ± 0.61

113–12

0.77 ± 0.14

3.67 ± 0.67

1.20 ± 0.17

3.87 ± 0.55

20–2

1.86 ± 0.41

8.86 ± 1.95

2.94 ± 0.31

9.48 ± 1.00

197–1

1.90 ± 0.47

9.05 ± 2.24

2.68 ± 0.44

8.65 ± 1.42

118–2

-

-

1.54 ± 0.22

4.97 ± 0.71

Hom.110–1

0.95 ± 0.14

4.52 ± 0.67

1.42 ± 0.30

4.58 ± 0.97