Skip to main content

Advertisement

Table 2 Calculated copy number for TSWV-N and nptII transgenes. For each line and for each gene system, the r line ratio (SQ trans /SQ end ) is shown together with its 95% confidence interval (δr line ) in the left column. The calculated copy number, obtained as the ratio between r line and the virtual calibrator r1 is shown in the right column, together with its 95% confidence interval.

From: Estimating the number of integrations in transformed plants by quantitative real-time PCR

  TSWV-N nptII
Line r line r line /r1 r line r line /r1
111–6 0.23 ± 0.04 1.10 ± 0.19 0.34 ± 0.05 1.10 ± 0.16
99–1 0.22 ± 0.05 1.05 ± 0.24 0.34 ± 0.07 1.10 ± 0.23
1–2 c 0.23 ± 0.04 1.10 ± 0.19 0.59 ± 0.05 1.90 ± 0.16
110–1 0.47 ± 0.07 2.24 ± 0.33 0.72 ± 0.06 2.32 ± 0.19
46–1 0.36 ± 0.12 1.71 ± 0.57 0.24 ± 0.09 0.77 ± 0.29
80–1 0.39 ± 0.05 1.86 ± 0.24 0.76 ± 0.13 2.45 ± 0.42
127–1 0.47 ± 0.11 2.24 ± 0.52 1.16 ± 0.13 3.74 ± 0.42
133–1 0.72 ± 0.10 3.43 ± 0.48 0.87 ± 0.09 2.81 ± 0.29
1–1 0.60 ± 0.13 2.86 ± 0.62 0.64 ± 0.12 2.06 ± 0.39
30–4 0.77 ± 0.19 3.67 ± 0.90 0.84 ± 0.19 2.71 ± 0.61
113–12 0.77 ± 0.14 3.67 ± 0.67 1.20 ± 0.17 3.87 ± 0.55
20–2 1.86 ± 0.41 8.86 ± 1.95 2.94 ± 0.31 9.48 ± 1.00
197–1 1.90 ± 0.47 9.05 ± 2.24 2.68 ± 0.44 8.65 ± 1.42
118–2 - - 1.54 ± 0.22 4.97 ± 0.71
Hom.110–1 0.95 ± 0.14 4.52 ± 0.67 1.42 ± 0.30 4.58 ± 0.97