Table 1 Bacterial Strains used in this work and their TFX phenotype (production, resistance, and sensitivity). Overproduction of TFX occurs when the TFX production is conferred using a multi-copy, broad host range plasmid such as pT2TFXK.
Strain | Characteristics | Reference |
|---|---|---|
Rhizobium | ||
T24 | R. leguminosarum bv. trifolii, TFX producing strain | 28 |
T24::Tn5-1 | TFX non-producing mutant, Tn5 insertion in tfxB | 10 |
CE3(pT2TFXK) | R. etli. Contains TFX-encoding plasmid, overproduces TFX | 20 |
CE3(pT2TX3K) | Plasmid contains tfxA deletion, non-TFX producing strain | 20 |
ANU794 | R. leguminosarum bv. trifolii, TFX-sensitive | 29 |
ANU794(pT2TX3K) | TFX-resistant | This work |
Agrobacterium vitis | ||
F2/5 | Biological control on grapevine, no control on other host-plant species, TFX-resistant | 30 |
F2/5(pT2TFXK) | Contains TFX-encoding plasmid, produces TFX, TFX-resistant | This work |
F2/5(pT2TX3K) | Plasmid contains tfxA deletion, non-TFX producing strain, TFX-resistant | This work |
CG49 | Tumorigenic, nopaline-type Ti plasmid, controlled by F2/5, sensitive to TFX overproduction | 31 |
CG78 | Tumorigenic, vitopine-type Ti plasmid, not controlled by F2/5 co-inoculation, sensitive to TFX overproduction | from TJ Burr |
K306 | Tumorigenic, octopine-type Ti plasmid, controlled by F2/5, sensitive to TFX overproduction | from TJ Burr |
CG107 | Tumorigenic, sensitive to TFX overproduction | from TJ Burr |
CG113 | Tumorigenic, TFX-sensitive | from TJ Burr |
CG435 | Tumorigenic, TFX-sensitive | from TJ Burr |