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Table 1 Bacterial Strains used in this work and their TFX phenotype (production, resistance, and sensitivity). Overproduction of TFX occurs when the TFX production is conferred using a multi-copy, broad host range plasmid such as pT2TFXK.

From: Expression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genes

Strain Characteristics Reference
Rhizobium   
T24 R. leguminosarum bv. trifolii, TFX producing strain 28
T24::Tn5-1 TFX non-producing mutant, Tn5 insertion in tfxB 10
CE3(pT2TFXK) R. etli. Contains TFX-encoding plasmid, overproduces TFX 20
CE3(pT2TX3K) Plasmid contains tfxA deletion, non-TFX producing strain 20
ANU794 R. leguminosarum bv. trifolii, TFX-sensitive 29
ANU794(pT2TX3K) TFX-resistant This work
Agrobacterium vitis   
F2/5 Biological control on grapevine, no control on other host-plant species, TFX-resistant 30
F2/5(pT2TFXK) Contains TFX-encoding plasmid, produces TFX, TFX-resistant This work
F2/5(pT2TX3K) Plasmid contains tfxA deletion, non-TFX producing strain, TFX-resistant This work
CG49 Tumorigenic, nopaline-type Ti plasmid, controlled by F2/5, sensitive to TFX overproduction 31
CG78 Tumorigenic, vitopine-type Ti plasmid, not controlled by F2/5 co-inoculation, sensitive to TFX overproduction from TJ Burr
K306 Tumorigenic, octopine-type Ti plasmid, controlled by F2/5, sensitive to TFX overproduction from TJ Burr
CG107 Tumorigenic, sensitive to TFX overproduction from TJ Burr
CG113 Tumorigenic, TFX-sensitive from TJ Burr
CG435 Tumorigenic, TFX-sensitive from TJ Burr