Effect of TFX production and inoculum ratio on Nicotiana glauca gall size. Legend: Gall diameter (mm) perpendicular to the stem was measured 1 month post-inoculation. Wound sites were inoculated with 5 μl of mixed bacterial suspensions. Mixtures were made immediately prior to inoculation. Each inoculum mixture was inoculated into three or four wounds on each of two plants, for a total of six to eight inoculations per treatment. Results that are significantly different at α = 0.05 are indicated with different letters within a group of inoculum mixtures. High ratios of F2/5 (pT2TFXK) :tumorigenic strain result in significant disease suppression for all three tested tumorigenic strains.