Figure 5

Gel filtration profiles. Gel filtration of purified and refolded Hep27scFv was performed on Superdex 75 column connected to a FPLC system pre-equilibrated with PBS. Sample volume and flow rate were 200 μl and 0.5 ml/min, respectively. Molecular mass was calibrated with ovalbumin (44 kDa), myoglobulin (17 kDa), and vitamin B12 (1.35 kDa).