SDS-PAGE and immunoblotting analysis of putified scFv. (A); SDS-PAGE stained with CBB. Lane M, protein molecular weight marker; lane 1, soluble proteins after refolding; lane 2, Flow-through fraction; lane 3, washed fraction; lane 4, eluted fraction. (B): Immunoblotting of purified Hep27scFv stained with mouse anti-His antibody (IgG) and alkaline phosphatase-conjugated anti-mouse IgG. Relative molecular mass (kDa) of standard proteins is shown on the left.