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Table 1 Percent signal change due to nucleotide substitution.

From: Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios

 

Relative percent change

Probe Name

MeC

MeU

1:1 DAP:A

AI936591

108

-38

27

AB029001

110

-7

112

U90544

113

-19

540

AA707570

115

-7

259

AK000445

116

22

23

AF054506

121

-36

47

AB007891

127

-16

117

D42045

138

62

35

AB006190

146

-28

481

AI935557

166

25

2

NM_006794

168

-20

1623

AB028946

168

-24

351

AK000601

179

51

451

AB020710

198

90

165

AK000527

230

-33

93

AI742085

246

5

41

AF017789

269

601

-44

AK001962

279

257

4

AF038661

291

-44

445

AB037797

301

295

279

AF035121

314

40

10

AF016369

332

324

149

AB034695

346

-22

363

U15932

377

66

43

AK001933

380

219

237

AJ223352

465

545

54

AF227899

559

106

603

AK000286

821

-57

922

AF052093

885

587

177

L77701

2356

1278

284

AL137493

4256

-37

2977

D86956

5999

2857

1367

  1. MeC and MeU substitution increases signal intensities for a small number of probes. Relative percent change was calculated by the equation: 100 * (modified sample signal - control sample signal) / control sample signal. Relative change for these probes due to 1:1 DAP:A substitution is also given as reference.
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BMC Biotechnology

ISSN: 1472-6750

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