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Figure 3 | BMC Biotechnology

Figure 3

From: Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios

Figure 3

Effects of DAP substitution are similar, and additive, to those seen with an increased hybridization time. (A) Comparison of intensities obtained with unmodified cRNA hybridized for 18 hours versus partially DAP-modified cRNA for 18 hours (blue data points) transposed on a comparison of intensities obtained with unmodified cRNA hybridized for 18 hours versus unmodified cRNA hybridized for 42 hours (orange data points). (B) Comparison of intensities obtained with unmodified cRNA hybridized for 18 hours versus partially DAP-modified cRNA hybridized for 42 hours. (C) Plot showing the relative increases with respect to the 18 hour, unmodified control for increased hybridization time (18 hour 42 hour), 1:1 DAP:A substitution, and a combination of increased hybridization time/DAP substitution. The total number of probes was divided into three equally sized bins according to their signal intensities, with the bins representing low, medium, and high expressers. Relative percent increases for each probe were derived from the equation: 100 * (modified sample signal [time, DAP, or combination of both] - control sample signal) / control sample signal, and are shown with standard deviation error bars.

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