Figure 2From: Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratiosModified purines and pyrimidines show differential effects on hybridization intensities when incorporated into cRNA targets. Modified and unmodified (Control) samples were normalized for concentration, fragmented, and hybridized onto Human Uniset I microarrays in duplicate, except for 5-methyl CTP samples, which were hybridized in duplicate onto arrays with 1100 probes spotted in 6-fold redundancy. Average signal intensities after background subtraction were plotted for all probes of the Human Uniset I array for the control samples versus (A) control, (B) fully DAP-substituted, (C) 1:1 substituted DAP, (D) fully meC-substituted, and (E) fully MeU-substituted.Back to article page