Figure 2

Development of antibody response following low-volume jet injection and particle bombardment. Injections performed at days 0, 14 and 28. Serum drawn prior to each injection and at days 42 and 56. Data presented for individual rabbits. Five rabbits (Rabbits 1–5) received DNA immunization by low-volume jet injection and five rabbits (Rabbits 6–10) received DNA immunization by particle bombardment. Antibody detected by ELISA and presented as optical density (O.D.). Serum dilution 1:250. A. Low volume jet injection. Statistically significant rises in antibody titer were detected at four serial time points in Rabbit 1 (light gray squares; ANOVA, P = 0.005) and three serial time points in Rabbit 2 (black squares; ANOVA, P < 0.001) as compared to negative control sera. B. Particle bombardment injection. Statistically significant rises in antibody titer were detected at two serial time points in Rabbit 6 (black squares; ANOVA, P < 0.001), Rabbit 7 (light gray squares; ANOVA, P < 0.001), and Rabbit 8 (medium gray squares; ANOVA, P 0 = 0.003) as compared to negative control sera. Rabbit 6 expired during anesthesia after the day 28 injection.