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Figure 3 | BMC Biotechnology

Figure 3

From: Cloning and characterization of a new β-Glucosidase from a metagenomic library of Rumen of cattle feeding with Miscanthus sinensis

Figure 3

Characterization of Unglu135B12. A. Characterization of Unglu135B12 by western blot. Lane 1. Crude extract of BL21 (DE3) carrying pET28a (+) vector; Lane 2: IPTG-uninduced crude extract of BL21 (DE3) carrying pET-Unglu135B12; Lane 3: IPTG-induced crude extract of BL21 (DE3) carrying pET-Unglu135B12; Lane 4: Eluted Unglu135B12 protein after purification by Ni-NTA His · Bind Resins; Lane 5: Dialyzed Unglu135B12 protein; Lane 6: Flow through; Lane M: protein Marker. B. Functional verification of purified Unglu135B12. 1. Crude extract of BL21 (DE3); 2: Crude extract of BL21 (DE3) carrying pET28a (+) vector; 3: IPTG-uninduced crude extract of BL21 (DE3) carrying pET-Unglu135B12; 4: IPTG-induced crude extract of BL21 (DE3) carrying pET-Unglu135B12; 5: Eluted Unglu135B12 protein after purification by Ni-NTA His · Bind Resins; 6: Dialyzed Unglu135B12 protein.

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BMC Biotechnology

ISSN: 1472-6750

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