Plasmids | Characteristics | Reference |
---|---|---|
pSLTS* | ori SC101(Ts) Ampr; ParaB for λ-Red; PtetR for I-SceI | This work |
pHA1887 | 1887 bp fragment of pUC19 that contains the replication origin and the bla gene conferring ampicillin resistance | |
pT2SC* | Ampr Cmr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene ligated into pHA1887. | This work |
pT2SK* | Ampr Kanr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene ligated into pHA1887. | This work |
pT2ST* | Ampr Trimr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene ligated into pHA1887. | This work |
pT2SCb* | Ampr Cmr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene that provides a bacterial consensus ribosomal binding site at the 3’-end ligated into pHA1887. | This work |
pASC | Ampr Cmr; a template plasmid for amplification of a selection cassette that includes an I-SceI site and a chloramphenicol resistance gene ligated into pHA1887. | This work |
pASK | Ampr Kanr; a template plasmid for amplification of a selection cassette that includes an I-SceI site and a kanamycin resistance gene ligated into pHA1887. | This work |
pAST | Ampr Trimr; a template plasmid for amplification of a selection cassette that includes an I-SceI site and a trimethoprim resistance ligated into pHA1887. | This work |
pTSC | Ampr Cmr; a template plasmid for amplification of a selection cassette that includes a transcription terminator, an I-SceI site and a chloramphenicol resistance gene ligated into pHA1887. | This work |
pTSK | Ampr Kanr; a template plasmid for amplification of a selection cassette that includes a transcription terminator, an I-SceI site and a kanamycin resistance gene ligated into pHA1887. | This work |
pTST | Ampr Trimr; a template plasmid for amplification of a selection cassette that includes a transcription terminator, an I-SceI site and a trimethoprim resistance gene ligated into pHA1887. | This work |
pKD46 | ori SC101(Ts) Ampr; ParaB for λ-Red | [1] |
pK-HT | ori SC101(Ts−) Ampr; Prha for λ-Red; PtetR for I-SceI | [2] |