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Table 1 Plasmids used in this work

From: A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica

Plasmids

Characteristics

Reference

pSLTS*

ori SC101(Ts) Ampr; ParaB for λ-Red; PtetR for I-SceI

This work

pHA1887

1887 bp fragment of pUC19 that contains the replication origin and the bla gene conferring ampicillin resistance

 

pT2SC*

Ampr Cmr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene ligated into pHA1887.

This work

pT2SK*

Ampr Kanr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene ligated into pHA1887.

This work

pT2ST*

Ampr Trimr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene ligated into pHA1887.

This work

pT2SCb*

Ampr Cmr; a template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene that provides a bacterial consensus ribosomal binding site at the 3’-end ligated into pHA1887.

This work

pASC

Ampr Cmr; a template plasmid for amplification of a selection cassette that includes an I-SceI site and a chloramphenicol resistance gene ligated into pHA1887.

This work

pASK

Ampr Kanr; a template plasmid for amplification of a selection cassette that includes an I-SceI site and a kanamycin resistance gene ligated into pHA1887.

This work

pAST

Ampr Trimr; a template plasmid for amplification of a selection cassette that includes an I-SceI site and a trimethoprim resistance ligated into pHA1887.

This work

pTSC

Ampr Cmr; a template plasmid for amplification of a selection cassette that includes a transcription terminator, an I-SceI site and a chloramphenicol resistance gene ligated into pHA1887.

This work

pTSK

Ampr Kanr; a template plasmid for amplification of a selection cassette that includes a transcription terminator, an I-SceI site and a kanamycin resistance gene ligated into pHA1887.

This work

pTST

Ampr Trimr; a template plasmid for amplification of a selection cassette that includes a transcription terminator, an I-SceI site and a trimethoprim resistance gene ligated into pHA1887.

This work

pKD46

ori SC101(Ts) Ampr; ParaB for λ-Red

[1]

pK-HT

ori SC101(Ts) Ampr; Prha for λ-Red; PtetR for I-SceI

[2]

  1. *These plasmids have been deposited to the addgene repository and are freely available for academic use.