TY - JOUR AU - McQualter, Richard B. AU - Somleva, Maria N. AU - Gebbie, Leigh K. AU - Li, Xuemei AU - Petrasovits, Lars A. AU - Snell, Kristi D. AU - Nielsen, Lars K. AU - Brumbley, Stevens M. PY - 2014 DA - 2014/09/10 TI - Factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass JO - BMC Biotechnology SP - 83 VL - 14 IS - 1 AB - Polyhydroxyalkanoates are linear biodegradable polyesters produced by bacteria as a carbon store and used to produce a range of bioplastics. Widespread polyhydroxyalkanoate production in C4 crops would decrease petroleum dependency by producing a renewable supply of biodegradable plastics along with residual biomass that could be converted into biofuels or energy. Increasing yields to commercial levels in biomass crops however remains a challenge. Previously, lower accumulation levels of the short side chain polyhydroxyalkanoate, polyhydroxybutyrate (PHB), were observed in the chloroplasts of mesophyll (M) cells compared to bundle sheath (BS) cells in transgenic maize (Zea mays), sugarcane (Saccharum sp.), and switchgrass (Panicum virgatum L.) leading to a significant decrease in the theoretical yield potential. Here we explore various factors which might affect polymer accumulation in mesophyll cells, including targeting of the PHB pathway enzymes to the mesophyll plastid and their access to substrate. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-14-83 DO - 10.1186/1472-6750-14-83 ID - McQualter2014 ER -