Table 1 In vitro selection scheme for selecting an androgen-dependent prostate cancer cell-specific scFv

1 (+)

LNCaP

1 × 10¹⁰

~8 × 10⁶

3

15

Panning

1 (−)

HGPIN

1 × 10¹⁰

~7 × 10⁶

0.5

15

Panning

BPH-1

~9 × 10⁶

0.5

BHPrE1

~7.5 × 10⁶

0.5

2 (+)

LNCaP

1 × 10¹⁰

~9 × 10⁶

2

15

Panning

2 (−)

HGPIN

1 × 10¹⁰

~8.5 × 10⁶

1

15

Panning

BPH-1

~9.5 × 10⁶

1

BHPrE1

~8 × 10⁶

1

3 (+)

LNCaP

1 × 10¹⁰

~9 × 10⁶

1

15

Panning

3 (−)

HGPIN

1 × 10¹⁰

~7 × 10⁶

2

15

Panning

BPH-1

~9 × 10⁶

2

BHPrE1

~8 × 10⁶

2

4 (+)

LNCaP

1 × 10⁷

1 × 10⁶

0.5

2.5

FACS

4 (−) a

HGPIN

1 × 10⁷

1 × 10⁶

0.5

1.5

FACS

4 (−) b

BPH-1

1 × 10⁷

1 × 10⁶

0.5

2

FACS

4 (−) c

BHPrE1

1 × 10⁷

1 × 10⁶

0.5

1.5

FACS

4 (−) d

PSMA

1 × 10⁷

1 nmol protein

0.5

1

FACS

5 (+)

LNCaP

1 × 10⁷

1 × 10⁶

0.5

5

FACS

6 (+)

LNCaP

1 × 10⁷

1 × 10⁵

0.5

2.75

FACS

6 (−) a

HGPIN

1 × 10⁷

1 × 10⁶

0.5

2.5

FACS

6 (−) b

BPH-1

1 × 10⁷

1 × 10⁶

0.5

2.5

FACS

6 (−) c

BHPrE1

1 × 10⁷

1 × 10⁶

0.5

2.5

FACS

6 (−) d

PSMA

1 × 10⁷

1 nmol protein

0.5

2

FACS

7 (+)

LNCaP

1 × 10⁷

1 × 10³

0.5

2.5

FACS