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Table 1 In vitro selection scheme for selecting an androgen-dependent prostate cancer cell-specific scFv

From: Identification of an antibody fragment specific for androgen-dependent prostate cancer cells

Selection round Target Yeast number Human cell number Incubation time (hours) Incubation volume (mL) Separation method
1 (+) LNCaP 1 × 1010 ~8 × 106 3 15 Panning
1 (−) HGPIN 1 × 1010 ~7 × 106 0.5 15 Panning
BPH-1 ~9 × 106 0.5
BHPrE1 ~7.5 × 106 0.5
2 (+) LNCaP 1 × 1010 ~9 × 106 2 15 Panning
2 (−) HGPIN 1 × 1010 ~8.5 × 106 1 15 Panning
BPH-1 ~9.5 × 106 1
BHPrE1 ~8 × 106 1
3 (+) LNCaP 1 × 1010 ~9 × 106 1 15 Panning
3 (−) HGPIN 1 × 1010 ~7 × 106 2 15 Panning
BPH-1 ~9 × 106 2
BHPrE1 ~8 × 106 2
4 (+) LNCaP 1 × 107 1 × 106 0.5 2.5 FACS
4 (−) a HGPIN 1 × 107 1 × 106 0.5 1.5 FACS
4 (−) b BPH-1 1 × 107 1 × 106 0.5 2 FACS
4 (−) c BHPrE1 1 × 107 1 × 106 0.5 1.5 FACS
4 (−) d PSMA 1 × 107 1 nmol protein 0.5 1 FACS
5 (+) LNCaP 1 × 107 1 × 106 0.5 5 FACS
6 (+) LNCaP 1 × 107 1 × 105 0.5 2.75 FACS
6 (−) a HGPIN 1 × 107 1 × 106 0.5 2.5 FACS
6 (−) b BPH-1 1 × 107 1 × 106 0.5 2.5 FACS
6 (−) c BHPrE1 1 × 107 1 × 106 0.5 2.5 FACS
6 (−) d PSMA 1 × 107 1 nmol protein 0.5 2 FACS
7 (+) LNCaP 1 × 107 1 × 103 0.5 2.5 FACS