Figure 2From: Construction of sized eukaryotic cDNA libraries using low input of total environmental metatranscriptomic RNAcDNA size fractions and libraries preparation from soil PL. Agarose gel separation of (A) amplified cDNA without size fractionation, (B) the three different cDNA fractions A (0.1–0.5 kb); B (0.5–1 kb) and C (1–4 kb) and (C) SfiI digested libraries PL-A, PL-B and PL-C. For each sized library, the size range of the cDNA inserts is similar before (B) and after (C) cloning.Back to article page