Table 3 The sequences and information of LAMP primers
Label | 5′pos | 3′pos | len | Tm | 5′dG | 3′dG | GCrate | Sequence |
|---|---|---|---|---|---|---|---|---|
F3 | 204 | 222 | 19 | 56.03 | -5.56 | -4.06 | 0.42 | ACCACAATCMACAGTCATT |
B3 | 398 | 415 | 18 | 55.58 | -7.53 | -4.56 | 0.50 | CCCGCATCATTTGATGTG |
FIP | Â | Â | 39 | Â | Â | Â | Â | CAAAGTCGCTTTGCCCTTGG-GATGCTATCTTCCGAAGGA |
BIP | Â | Â | 41 | Â | Â | Â | Â | GATCAAACGGCCTGCACAAG-GRAATGTCATTTTGCT RAATG |
F2 | 243 | 261 | 19 | 55.33 | -5.14 | -4.71 | 0.47 | GATGCTATCTTCCGAAGGA |
F1c | 291 | 310 | 20 | 61.82 | -4.16 | -5.45 | 0.55 | CAAAGTCGCTTTGCCCTTGG |
B2 | 377 | 397 | 21 | 55.47 | -4.51 | -4.07 | 0.38 | GR(G or A)AATGTCATTTTGCTRAATG |
B1c | 326 | 345 | 20 | 61.55 | -3.92 | -4.66 | 0.55 | GATCAAACGGCCTGCACAAG |
LF | 266 | 286 | 21 | 61.30 | -6.24 | -5.45 | 0.48 | TGCGTTGGTTCAATTCTTGGG |
LB | 346 | 367 | 22 | 60.11 | -5.26 | -3.73 | 0.45 | GACGTCTTACAACGCAGTAACT |